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文献和实验NS5A Phosphorylation and Hyperphosphorylation
NS5A phosphorylation can be studied in two ways: in living cells and in vitro. The former has several advantages: NS5A phosphorylation takes place in a cellular background and therefore might mimic more closely the real in vivo situation
Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies
HCV is a small positive-strand RNA virus responsible for a considerable proportion of acute and chronic hepatitis in humans. Although all HCV enzymes are, in theory, equally appropriate for therapeutic intervention, the NS3-NS4A serine
Expression and Characterization of the HCV NS2 Protease
and cell-free expression systems have shown that host signalase is responsible for cleavages between the structural proteins and at the N-terminus of NS2 (1 –4 ), whereas a chymotrypsin-like serine protease located in the N-terminal region of NS
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