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文献和实验Synthesis of Oligopeptides Containing an Oxirane Ring in the Place of a Peptidic Bond
(up to a three-peptide) containing an epoxide in the place of the peptide bond. The structures prepared here can be identified, using the notation suggested by Spatola ( 12 ) as AAxψ[ traws -epoxy]-AAy. The general synthetic approach described in this chapter is based
Testing Rabbit Bleeds By Elisa
as a solid if soluble. Mix, adding glutaraldehyde last. Peptide and BSA turn a little yellow even before adding glutaraldehyde. Incubate 90'' at 37 deg C. Add 0.1 volumes of 0.1 M NaBH4 in 0.1 M NaHCO3. There will be some bubbling. Add
A Method for Assaying Deubiquitinating Enzymes
) have constructed Ub-αNH-peptide extensions containing "PEST" sequences. Of these, Ub-PESTc contains a peptide extension of 18 amino acids, which carries a single tyrosine residue that can be radioiodinated and is short enough to be released as an acid-soluble
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