5(Z),8(Z),14(Z)-Eicosatrienoic

E.Z.N.A. Cycle-Pure Kit Spin Protocol

from 100bp to 500bp gives yields of 60%- 90%. The ratio of (A )/280 (A ) is an indication of nucleic acid purity. Alternatively, yield (as well as quality) can sometimes be best determined by agarose gel/ethidium bromide electrophoresis.

E. Z. N.A.TM X-press Plasmid Protocol

1.5 and 2.0 mL Centrifuge Tubes 3. Vortex 实验步骤   1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate selective antibiotic. Incubate 14-16 hours at 37℃

E-Z 96 X-press Plasmid Vacuum Protocol

with clean absorbent paper towels. 13. Place the E-Z 96 X-press Plate back to the vacuum manifold and apply the maximum vacuum for another 10 minutes. This step will evaporate any remaining ethanol from membrane. 14. Turn off the vacuum. Assemble