3(Z),6(Z),9(Z),12(Z),15(Z)-Oct

E.Z.N.Z.TM MicroElute RNA Clean-up Protocol

实验步骤   1. Measure the volume of sample and adjust the sample volume to 100ul with DEPC-Water and proceed to step 2. 2. Add 350ul QVL Lysis buffer and mix by vortexing at maximum speed for 15 seconds. When process small

E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol

. Discard the flow-through and the collection tube. 8. Elution of RNA. Transfer the column to a clean 1.5 ml microfuge tube (not supplied with kit) and pipet 15-30 ul of DEPC-treated water (supplied with kit) into the column. Make sure to add water

Lac-Z Detection in Tail Biopsies

2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X PBS solution over biopsy with 2.5% Glutaraldehyde diluted in SpM buffer