Recombinant E.Coli GroES (HSP1

Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector

of the Autographa californica nuclear polyhedrosis virus (AcNPV) are abundantly expressed during the late stages of infection. The recombinant proteins are usually soluble and functionally similar to their authentic counterparts (l –7 ). In the following sections

Maxiprep of plasmid DNA from E.coli protocol

  Solutions/reagents: LB broth + selective marker 50% sterile glycerol TEG (25mM Tris-Cl, 10mM EDTA, 50mM dextrose) 20 mg/ml lysozyme 10% SDS 4M NaOH autoclaved water Solution 3 (3M potassium-acetate, 2M acetic acid

E.coli genomic library construct 求教

） 5. ethanol 沉淀，electrotransformation, 结果得到的colony比较少，0.5ug 连接产物沉淀后, dissolve in 20ul ddH2O, 2ul + 50ul cell, spread on 8 plates, 总共只得到约1000个colonies（由于vector是单酶切，这里面还有载体自连产物，电转感受态细胞的转化效率很高，10的7次方－10的8次方），我的目的是希望尽可能cover E.coli 2kb-5kb之间的gene，所以希望能够得到5000－1w左右