Glucose Colorimetric Assay Kit

Cayman葡萄糖测定法是一种简单、可重复且灵敏的测定血浆、血清和尿液中葡萄糖的工具。葡萄糖测定法使用葡萄糖氧化酶-过氧化物反应来测定葡萄糖浓度。在该测定法中，葡萄糖被氧化为d-葡萄糖酸内酯，同时黄素腺嘌呤二核苷酸(FAD)依赖性酶葡萄糖氧化酶被还原。还原形式的葡萄糖氧化酶被分子氧再生为其氧化形式，产生过氧化氢。最后，在辣根过氧化物酶的催化剂下，过氧化氢与3,5-二氯-2-羟基苯磺酸和4-氨基安替比林反应，生成粉红色染料，其最佳吸收波长为514nm。Cayman's Glucose Assay provides a simple, reproducible, and sensitive tool for assaying glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. In this assay, glucose is oxidized to d-gluconolactone with concomitant reduction of the flavin adenine dinucleotide (FAD)-dependent enzyme glucose oxidase. The reduced form of glucose oxidase is regenerated to its oxidized form by molecular oxygen to produce hydrogen peroxide. Finally, with horseradish peroxidase as a catalyst, hydrogen peroxide reacts with 3,5-dichloro-2-hydroxybenzenesulfonic acid and 4-aminoantipyrine to generate a pink dye with an optimal absorption at 514 nm.