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文献和实验Expression of Recombinant Membrane-Type MMPs
” (1 ) there has been much interest in the production of recombinant protein to facilitate both structural and functional analyses. The proteolytic activity of the membrane-type-1 MMP (MT1-MMP) has been studied using recombinant material produced from expression in E. coli (2 ,3 ). These data
Production and Crystallization of Bacterial Type V Secretion Proteins
and the production of diffracting crystals. Since the type V secretion pathway involves unusually large substrate proteins (passenger domains or TpsA) and membrane proteins (β-barrel domains or TpsB), crystallography of type V secretion proteins deals with additional
Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3
Adenoviruses have become a popular vehicle for gene transfer into animal and human cells. However, wide prevalence of preexisting immunity to human adenovirus (HAdV) and the promiscuous nature of the virus have made the use of nonhuman
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