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- 10007904-1 mg
- 2025年07月08日
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文献和实验reached 1.0, isopropyl-1-thio-β-d-galactopyranoside was added to a final concentration of 1 mM. After 5 h of induction, the cells were harvested by centrifugation at 4,000 × g for 20 min. 样品处理 For the purification of protein from 2 liters of culture
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
bacterial expression vectors. MATERIALS Buffers, Solutions, and Reagents Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) Isopropyl-β-D-thio-galactoside (IPTG) LB broth, containing appropriate antibiotic
Nested Deletions Using Exonuclease-III and Mung Bean Nuclease
than using a 3'' overhang: Cut with the enzyme to be filled in Heat 70° C for 15 min. Quench on ice. Add thio-d NTP to 40uM and 5U klenow 20 min at RT. Heat to 65°C for 10min. Phenol/CHCL3 extract
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