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文献和实验The study of cellular function of a particular gene can be advanced by either specifically eliminating or blocking the function of its gene product. Although antisense RNA has frequently been used for this purpose, the hammerhead ribozyme
Gel-elongation assay for type II fatty acid synthesis
1 Determination of target selectivity E. coli fatty acid synthesis (FASII) gel-elongation assay used 4 µM of [2-14 C]malonyl-CoA (60 mCi/mmol) and 20 µM of acetyl-CoA as substrates. The reaction was carried out at 37°C for 30
Gel-elongation assay for type II fatty acid s
Gel-elongation assay for type II fatty acid synthesis Srinivas Kodali Andrew Galgoci Sheo Singh Dr. Jun Wang Dr., jun_wang2@merck.com, Merck Research Laboratories
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