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文献和实验Characterization of Melanocortin Receptors
CHO‐K1 cells expressing a recombinant melanocortin receptor (MC3R, MC4R and MC5R membranes are available from Perkin Elmer Life Sciences) Culture medium (see recipe
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
a method to evaluate the effect that small molecules have on the binding interactions of a nuclear receptor protein with a series of peptides. The multiplexed microsphere?based system employs peptide?coupled microsphere populations that are fluorescently
Blocking of unwanted non-specific staining
and other cell types. By exploiting the preferential avidity of Fc receptor for human > mouse Ig> rabbit > swine > goat immunoglubulins, one may use a blocking of the receptors with a reagent which will not interfere with the secondary reagents
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