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- 2025年07月08日
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文献和实验Utilizing Peptide SPOT Arrays to Identify Protein Interactions
of synthesized peptides 12? to 18?amino acids long, with overlapping sequences that cover the entire sequence of a protein, covalently linked to a solid support. This unit describes how to construct peptide SPOT arrays, biotinylate recombinant proteins
Synthesis and Probing of Membrane-bound Peptide Arrays
can be made. For the introduction of randomized X positions in the peptide sequences, take the appropriate Fmoc-AA-mix stock solution from the freezer, bring to room temperature, and activate by adding DIC (1.5 µl per 100 µl-vial
with dye-labeled compounds is used to determine crucial experimental parameters including spotting concentration, spotting solution, immobilization efficiency, and blocking conditions even in cases where recommendations from the slide manufacturer
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