Anti-Myc; 100 μg

Anti-Myc

c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression.

• Transcription factor p64
• avian myelocytomatosis viral oncogene homolog
• myc proto-oncogene protein
• v-myc avian myelocytomatosis viral oncogene homolog
• v-myc myelocytomatosis viral oncogene homolog (avian)

Recognizes Myc isoforms Mr 50-67kDa.

• Chromatin Immunoprecipitation (ChIP)
• Immunocytochemistry
• Immunoprecipitation
• Western Blotting

Immunoprecipitation:
4 µg of a previous antibody immuno-precipitated Myc as shown by an independent laboratory.

Immunocytochemistry:
This antibody has been used for immunocytochemistry by an independent laboratory. (Ohtsuka, T., 1998)

• Human
• Mouse
• Avian
• Xenopus

Strongest reactivity observed with human and mouse Myc. Will also cross-react with avian and Xenopus.

IgG

Bacterially expressed fusion-protein corresponding to the full-length human Myc.

67 kDa

EGF-stimulated A431 cells, normal human colon, HeLa whole cell lysate or Jurkat whole cell lysate.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.

Purified rabbit polyclonal IgG in buffer containing PBS, 0.05% sodium azide with 2 mg/mL BSA. Frozen solution.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• MYC
• C-MYC
• c-Myc

Routinely evaluated by western blot on A431 lysates from EGF-stimulated human A431 cells, human A431 cells and HeLa nuclear extract.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Myc in A431 lysates from EGF-stimulated human A431 cells. 0.5-2 µg/mL of a previous lot detected Myc in RIPA lysates from human A431 cells and HeLa nuclear extract.

Epigenetics & Nuclear Function

Transcription Factors

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Upstate (Millipore)

0.5 mg/mL

Polyclonal Antibody

Purified

Rabbit