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- 详细信息
- 技术资料
- 应用范围:
Flow Cytometry;Immunoprecipitation
- 宿主:
Mouse
- 抗原来源:
0
- 库存:
大量
- 适应物种:
Human;Mouse
- 是否单克隆:
1
- 规格:
200 μg
Product Images
IB: Jurkat cell lysate was probed with anti-CD3ε(0.5μg/ml).
T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | FC, IP, WB, IC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CD3ε, clone APA1/1
Replaces:
CBL1302
Specificity:
CDε
Key Applications:
- Flow Cytometry
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
Application Notes:
Clone APA1/1 has been demonstrated to recognize a site on CD3&epsilon: that is exposed upon engagement of the T-Cell Receptor complex. Exposure of this neoepitope precedes CD3 phosphorylation and recruitment and activation of ZAP-70, which initiates the signaling cascade produced by T-cell activation. As such, clone APA1/1 provides the earliest known marker for T-Cell Receptor engagement, and thus T-cell activation.
Species Reactivity:
- Human
- Mouse
Isotype:
IgG1
Immunogen:
Purified human CD3 epsilon
Molecular Weight:
~23kDa
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
Gene Symbol:
- CD3E
- T3E
- TCRE
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Antibody Category:
Inflammation & Immunology
Antibody Sub-Category:
Immunoglobulins & Immunology
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Format:
Purified
Host:
Mouse
Antibody Type:
Monoclonal Antibody
Product Family Information
CD3 AntibodiesMillipore’s phosphoserine antibody demonstrates specificity against Phosphoserine. See below for data and references for Millipore’s phosphoserine antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Purified human CD3 epsilon 详细描述见链接:http://www.millipore.com/catalogue/item/05-785
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Anti-CD3ε, clone APA1/1; 200 μg
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