Anti-CD3ε, clone APA1/1; 200 μg

Anti-CD3ε, clone APA1/1; 200 μ

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  • 询价
  • 05-785
  • 2025年07月12日
  • Flow Cytometry;Immunoprecipitation
  • Mouse
  • Human;Mouse
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    • 详细信息
    • 技术资料
    • 应用范围

      Flow Cytometry;Immunoprecipitation

    • 宿主

      Mouse

    • 抗原来源

      0

    • 库存

      大量

    • 适应物种

      Human;Mouse

    • 是否单克隆

      1

    • 规格

      200 μg

    Product Images

    IB: Jurkat cell lysate was probed with anti-CD3ε(0.5μg/ml).

    T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue...

    Species Reactivity Key Applications Host Format Antibody Type
    H, M FC, IP, WB, IC Mouse Purified Monoclonal Antibody
    Description:
    Anti-CD3ε, clone APA1/1
    Replaces:
    CBL1302
    Specificity:
    CDε
    Key Applications:
    • Flow Cytometry
    • Immunoprecipitation
    • Western Blotting
    • Immunocytochemistry
    Application Notes:
    Clone APA1/1 has been demonstrated to recognize a site on CD3&epsilon: that is exposed upon engagement of the T-Cell Receptor complex. Exposure of this neoepitope precedes CD3 phosphorylation and recruitment and activation of ZAP-70, which initiates the signaling cascade produced by T-cell activation. As such, clone APA1/1 provides the earliest known marker for T-Cell Receptor engagement, and thus T-cell activation.
    Species Reactivity:
    • Human
    • Mouse
    Isotype:
    IgG1
    Immunogen:
    Purified human CD3 epsilon
    Molecular Weight:
    ~23kDa
    Presentation:
    0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
    Storage Conditions:
    2 years at -20°C
    Gene Symbol:
    • CD3E
    • T3E
    • TCRE
    Quality Assurance:
    routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
    Antibody Category:
    Inflammation & Immunology
    Antibody Sub-Category:
    Immunoglobulins & Immunology
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Format:
    Purified
    Host:
    Mouse
    Antibody Type:
    Monoclonal Antibody

    Product Family Information

    CD3 Antibodies

    Millipore’s phosphoserine antibody demonstrates specificity against Phosphoserine. See below for data and references for Millipore’s phosphoserine antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon.

    » More…


    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    Purified human CD3 epsilon 详细描述见链接:http://www.millipore.com/catalogue/item/05-785

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    Anti-CD3ε, clone APA1/1; 200 μg
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