- 询价
- 05-785
- 2025年07月12日
- Flow Cytometry;Immunoprecipitation
- Mouse
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Flow Cytometry;Immunoprecipitation
- 宿主：
Mouse
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Human;Mouse
- 是否单克隆：
1
- 规格：
200 μg
Product Images
IB: Jurkat cell lysate was probed with anti-CD3ε(0.5μg/ml).
T-cell Activation by an Antigen presenting Cell. Panel A is an image of a single Antigen-Presenting Cell (APC; a Raji cell, stained blue...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | FC, IP, WB, IC | Mouse | Purified | Monoclonal Antibody |
- Flow Cytometry
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
- Human
- Mouse
- CD3E
- T3E
- TCRE
Product Family Information
CD3 AntibodiesMillipore’s phosphoserine antibody demonstrates specificity against Phosphoserine. See below for data and references for Millipore’s phosphoserine antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Purified human CD3 epsilon 详细描述见链接：http://www.millipore.com/catalogue/item/05-785
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文献和实验Dual- and Triple-Color Fluorospot
of 35% ethanol for 1 min at room temperature and wash the plate five times with 200 μl of sterile water per well. b. Mix coating mAbs for IFN-γ and IL-2 in the same tube in sterile PBS, pH 7.4, at a final concentration of 15 μg/ml each. Add
T-Cell Activation Using mAb to CD3
of spleen cells gives a good response. If experimental conditions require, a titration of cell densities (2-3x106 /ml to 105 /ml) should be performed for optimization. After washing the wells with PBS (step 6 above), add 200µl of the cell suspension
：1）正常对照DNA杂交浓度达50μg/μl时显蓝色，RNA杂交浓度需100～200μg/μl时显蓝色，随浓度上升，斑点蓝色加深，KD患者外周血单个核细胞 （PBMC）经anti-CD3刺激体外培养后，RNA杂交浓度需500～1000μg/μl时方显蓝色，当IVIG治疗后或PBMC体外培养加入IL-6 McAb时，RNA杂交浓度达200μg/μl可显蓝色，与正常对照组比较，KD患者外周血淋巴细胞 P53基因mRNA表达水平降低，当IVIG治疗后或PBMC体外培养加入IL-6 McAb时，淋巴
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