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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 供应商:
上海研卉生物科技有限公司
- 英文名:
Enhanced Apoptotic DNA Ladder Detection Kit
- 库存:
大量
- 规格:
50次分析
Kit Summary:
• Detection method- Agarose gel electrophoresis
• Sample type- DNA from all cell types and tissues undergoing apoptosis
• Species reactivity- Mammalian
• Kit size- 50 assays
• Applications- Detects efficiently DNA fragmentation from apoptotic cells
Features & Benefits:
• Simple one-step procedure; takes only 90 minutes
• Fast and convenient
• Higher sensitivity in comparison to other similar kits in the market. The assay can be used to detect apoptotic DNA ladder in both tissues and cells.
Kit components:
• TE Lysis Buffer
• Enzyme B (Lyophilized)
• Ammonium Acetate Solution
• DNA Suspension Buffer
• Staining Dye (10000X)
Description:
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Enhanced Apoptotic DNA Ladder Detection Kit provides an easy and sensitive means for detecting DNA fragmentation in apoptotic cells. Unlike other commercially available kits that require 1-2 days to perform the procedure, the new detection method requires less than 90 minutes to prepare DNA, with neither extraction nor using columns. DNA fragmentation can be easily visualized by agarose gel electrophoreses stained with a highly sensitive dye.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
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文献和实验一、反应曲线 首先,我们了解一下化学反应的时间与吸光度一个曲线图,可以划分为四个区:延迟区、等速区、过渡区和平衡区,如下图所示: 二、反应原理 对于延迟区来讲,无规律可寻,对于指标检测没有意义。 等速区对应的指标检测方法是速率法。速率法又称连续监测法,是在测定酶活性或用酶法测定代谢产物时,连续选取时间-吸光度曲线中线性期内4个以上测光点作为读数点,并以单位时间吸光度变化值计算结果。线性期就是此期间内各测光点之间的吸光度差值相等,此线性期对酶促反应的底物来说属于零级反应。其测光点一般设置在加入
例一 gongyulai :我用的是药物诱导癌细胞凋亡,做DNA Ladder(用的是北京鼎国的动物细胞凋亡梯子提取试剂盒)。跑出来的电泳不是一条条带,而是每条很长的拖尾现象,Marker到是出来了。我是按说明书上0。8%的琼脂糖。我用的是60v电压。不知道什么原因?郁闷。说明书上说混合温和是什么意思,我又没用力摇。我还想问收集凋亡细胞时离心时转速多少比较好?chujun_hust :(1)“跑出来的电泳不是一条条带,而是每条很长的拖尾现象”: 可能是由于你点样时,时间太长了,导致样品扩散
EDTA) for 10 minutes at 100 V. 2) To 3 μg (3 μl) of ladder, add loading buffer (recommended final loading buffer concentration is 20 mM MOPS; pH 7.0, 6% w/v formaldehyde, 31% v/v deionized formamide, 1 mM EDTA, 0.0125% xylene cyanol
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