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- 详细信息
- 文献和实验
- 技术资料
- 宿主:
其它
- 应用范围:
western blot
- 适应物种:
大鼠
- 供应商:
上海研卉生物
- 抗原来源:
其它
- 库存:
大量
- 是否单克隆:
0
- 抗体英文名:
Anti-Rat GM-CSF
- 目录编号:
500-P225
- 规格:
100ug
Anti-Rat GM-CSF
ELISA
SandwichTo detect Rat GM-CSF by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat GM-CSF (500-P225Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.
Western Blot
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of Rat GM-CSF (0.5ng/ml), a concentration of 0.09–0.14 μg/ml of this antibody is required.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
Anti-factor Xa (Anti-Xa) Assay
The anti-factor Xa (anti-Xa) assay is a functional assay that facilitates the measurement of antithrombin (AT)-catalyzed inhibition of factor Xa by unfractionated heparin (UFH) and direct inhibition of factor Xa by low-molecular-weight
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
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