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- 2025年10月03日
- Flow cytometry
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
FITC Mouse Anti-Human Cyclin D1 Antibody Set
- 宿主：
Mouse
- 库存：
大量
- 保存条件：
低温
- 供应商：
上海研卉生物科技有限公司
- 抗原来源：
Goat，Rabbit，Mouse
- 保质期：
一年
- 抗体英文名：
FITC Mouse Anti-Human Cyclin D1 Antibody Set
- 是否单克隆：
多克隆
- 适应物种：
人
- 应用范围：
Flow cytometry
- 规格：
100T
Cyclin D1，即G1/S特异性周期蛋白D1，设计特异性识别Cyclin D1的抗体，偶联合适的荧光素，即可检测到Cyclin D1在细胞周期里的变化。
研究Cyclin D1在细胞周期调控中的作用，从而找出治疗癌症的的药物。
实验要点：1 需固定破膜
2需做同型对照
3建议将WI-38人成纤维细胞ATCC CCL-75做为阳性对照。
Description
Cyclins and cyclin-dependent kinases (cdks) are subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle. Specific substrates for cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. Human cyclin D1 migrates at a molecular weight of 36 kDa in SDS/PAGE.
Clone G124-326 recognizes human Cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human Cyclin D1 was used as immunogen. The G124-326 antibody was initially characterized by immunoprecipitation and western blot analysis. In publications it has been used for western blot analysis, flow cytometric analysis, and immunohistochemistry of frozen tissue sections.
Clone MOPC-21 is a mouse IgG1 isotype (negative) control. The MOPC-21 antibody has unknown specificity. The G124-326 and MOPC-21 FITC conjugates are matched in F/P ratios. The optimum F/P ratio was experimentally determined by flow cytometric analysis.
Components
| Part No. | Description | Size |
|---|---|---|
| 51-13854X-2 | FITC Mouse IgG1, κ Isotype Control | 100 Tests (1 ea) |
| 51-13864X | FITC Mouse Anti-Human Cyclin D1 | 100 Tests (1 ea) |
Suggested Companion Products
| Cat No. | Description | Size | |
|---|---|---|---|
| 554180 | Purified Mouse Anti-Human Cyclin D1 G124-326 RUO | 0.1 mg |
|
| 554181 | Purified Mouse Anti-Human Cyclin D1 G124-326 RUO | 0.25 mg |
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E, and is based on protocols originally designed by Z. Darzynkiewicz and colleagues. Although FITC-conjugated
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