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- 文献和实验
- 技术资料
- 应用范围:
Western Blotting;Immunohistochemistry
- 宿主:
Mouse
- 抗原来源:
0
- 适应物种:
All
- 库存:
大量
- 是否单克隆:
1
- 规格:
200 μg
Product Images
WB: 3T3 cell lysate containing 5 nanograms of recombinant luciferase probed with anti-Luciferase (0.5 micrograms/ml).
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | WB, IH | Mouse | Purified | Monoclonal Antibody |
- Western Blotting
- Immunohistochemistry
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Full-length recombinant luciferase from Photinus pyralis. 详细描述见链接:http://www.millipore.com/catalogue/item/05-603
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
mg/ml. Store Abs at -20o C or further concentrate by using Centriprep. And store at -20o C. Regenerate Ab column with Regenerate buffer (~200 ml). 上一篇:Purification Of Anti Peptide Antibod 下一篇:抗体检测及杂交瘤细胞的选择
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
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