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Mouse抗IKKβ单克隆抗体 WB适用 ikb激酶2

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  • 询价
  • 05-535
  • 2025年08月24日
  • Western Blotting
  • Mouse
  • Human;Mouse;Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Western Blotting

    • 宿主

      Mouse

    • 适应物种

      Human;Mouse;Rat

    • 抗原来源

      0

    • 库存

      大量

    • 是否单克隆

      1

    • 规格

      100 μg

    Product Images

    WB: Jurkat cell lysate was probed with Anti-IKKβ/IKK2, clone 10AG2

    Western Blot Analysis:
    HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IKKβ/IKK2
    (2...

    Species Reactivity Key Applications Host Format Antibody Type
    H, R, M WB Mouse Purified Monoclonal Antibody
    Description:
    Anti-IKKβ, clone 10AG2
    Background Information:
    IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1.
    Alternate Names:
    • I-kappa-B kinase 2
    • I-kappa-B-kinase beta
    • Nuclear factor NF-kappa-B inhibitor kinase beta
    • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
    • inhibitor of nuclear factor kappa B kinase beta subunit
    Clone:
    10AG2
    Specificity:
    This antibody recognizes IKKβ/IKK2, Mr 87 kDa.
    Key Applications:
    Western Blotting
    Species Reactivity:
    • Human
    • Rat
    • Mouse
    Species Reactivity Note:
    Proven to react with human, mouse and rat. Other species not tested.
    Isotype:
    IgG1
    Immunogen:
    His-tagged, full length human IKKβ/IKK2. Clone: 10AG2.
    Molecular Weight:
    87 kDa
    Control:
    Jurkat cell lysate and human cervical carcinoma.
    Included Positive Antigen Control: Catalog # 12-303, Jurkat cell extract. Use 20 µg per lane for minigels.
    Presentation:
    Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.05% BSA, with 0.05% sodium azide. Frozen solution.
    Storage Conditions:
    Stable for 1 year at -20ºC from date of receipt.
    Gene Symbol:
    • IKBKB
    • IKK-beta
    • NFKBIKB
    • IKK2
    • FLJ40509
    • IKKB
    • MGC131801
    • IKK-B
    • IkBKB
    Quality Assurance:
    Routinely evaluated by western blot on human Jurkat RIPA cell lysate, human A431, HeLa nuclear extract, mouse 3T3/A31 or rat PC-12 RIPA cell lysates.

    Western Blot Analysis:
    0.5-2 µg/mL of this lot detected IKK/IKK2 in human HeLa cell lysate. A previous lot was tested in human Jurkat RIPA cell lysate, A431, extract, mouse 3T3/A31 and rat PC-12 RIPA cell lysates.
    Antibody Category:
    Signaling
    Antibody Sub-Category:
    Immunological Signaling
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Concentration:
    0.5 mg/mL
    Antibody Type:
    Monoclonal Antibody
    Format:
    Purified
    Host:
    Mouse

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    His-tagged, full length human IKKbeta/IKK2 详细描述见链接:http://www.millipore.com/catalogue/item/05-535

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