Anti-Phosphotyrosine, 4G10<sup

>®</sup> Platinum; 100 μL

价格询价

货号05-1050
更新日期2026年05月29日

应用范围Western Blotting;Immunofluorescence
宿主Mouse
适应物种All

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详细信息
文献和实验
技术资料

应用范围：
Western Blotting;Immunofluorescence
宿主：
Mouse
库存：
大量
适应物种：
All
抗原来源：
0
是否单克隆：
1
规格：
100 μL

Lysates from various cells with various treatments to stimulated varying tyrosine phosphorylations on multiple substrates were resolved in parallel by SDS-PAGE and transferred to PVDF membrane. The membranes were probed with one of three different anti-phosphotyrosine antibodies, 4G10® (Cat. 05-321), PY20 (Cat. No. 05-947), or 4G10® Platinum (Cat. No. 05-1050). The various blots show the advantages of the anti-phosphotyrosine monoclonal cocktail, 4G10® Platinum.

4G10® Platinum, Anti-Phosphotyrosine IHC(P) staining of various cancer tissue samples.

(05-1050 LN: R0709B0207, 05-1050X LN: R0709B0208)
EGF-stimulated A431 cell lysate (Cat. No.12-302) was resolved by SDS-PAGE and transferred to PVDF membrane. PCDF was probed with 4G10® Platinum, anti-phosphotyrosine (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Species Reactivity	Key Applications	Host	Format	Antibody Type
A	WB, IC, ELISA, IH, IF, FC, IP, IPK	Mouse	Purified	Monoclonal Antibody

Description:

4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b)

Background Information:

The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.

Specificity:

Tyrosine-phosphorylated proteins from all species

Key Applications:

Western Blotting
Immunocytochemistry
ELISA
Immunohistochemistry
Immunofluorescence
Flow Cytometry
Immunoprecipitation
IP-Kinase Assay

Application Notes:

Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.

Species Reactivity:

Isotype:

IgG2b

Immunogen:

4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein

Modifications:

Phosphorylation

Molecular Weight:

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Control:

Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.

Presentation:

Proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide.
Liquid at 2-8°C.

Storage Conditions:

Stable for 1 year at from date of receipt.

Quality Assurance:

Evaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

Antibody Category:

Signaling

Antibody Sub-Category:

General Post-translation Modification

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Trade Name:

Upstate (Millipore)

Format:

Purified

Host:

Mouse

Antibody Type:

Monoclonal Antibody

Product Family Information

Phosphotyrosine Signaling Solutions

» More…

Phosphotyrosine Antibodies

Millipore’s phosphotyrosine antibody demonstrates specificity against Phosphotyrosine. See below for data and references for Millipore’s phosphotyrosine antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon.

» More…

更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

4G10^® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein. 详细描述见链接：http://www.millipore.com/catalogue/item/05-1050

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