- ￥1392
- LSM BIO
- 进口
- PVT20937
- 2025年07月10日
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- 文献和实验
- 技术资料
- 保存条件：
负20℃
- 保质期：
3个月
- 英文名：
pBluescriptR-RANGAP1
- 库存：
50
- 供应商：
LSM BIO
- 规格：
2ug
pBluescriptR-RANGAP1
PVT20937 2ug
Product Name:pBluescriptR-RANGAP1
Replicon: pUC
Prokaryotic resistance: Amp
Growth Strain(s): DH5a
Host: Escherichia coli
Vector type: Gene template
Fragment type: cDNA
species: Human
Prokaryotic resistance: Amp
Resistance Marker:
Fluorescent labeling:
*For Lab research use only
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文献和实验An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage
and purifi ed YFP-SUMO-1 and CFP-RanGAP1 as model substrates that are covalently coupled in the presence of recombinant SUMO E1 and E2 enzymes and ATP. Reactions of 25 μl volume, set up in 384-wells plates, give suffi cient signal for analysis. Consequently
Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
for in vitro sumoylation of RanGAP1 and Sp100 in the absence or presence of the SUMO E3 ligase RanBP2, respectively, are provided. Both protocols can be adapted easily to test in vitro conjugation of other target proteins and/or E3 ligases.
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