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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Lophura leucomelanos Lysozyme C (LYZ)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Lophura leucomelanos Lysozyme C (LYZ)品名简称:
Recombinant Lophura leucomelanos LYZ protein货号:
CSB-EP013283LIQ规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
LYZUniprot No.:
P24364别名:
1,4-beta-N-acetylmuramidase种属:
Lophura leucomelanos (Kalij pheasant)蛋白长度:
Full Length来源:
E.coli分子量:
30.3 kDa表达区域:
1-129aa氨基酸序列:
KVYGRCELAAAMKRLGLDNYRGYSLGNWVCAAKYESNFNTHATNRNTDGSTDYGILQINSRWWCNDGKTPGSRNLCHIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVSVWTRGCRL蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.功能2:
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.文献:
"The amino acid sequence of lysozyme from kalij pheasant (Lophura leucomelana) egg-white."Araki T., Kudo K., Kuramoto M., Torikata T.Agric. Biol. Chem. 55:1701-1706(1991)研究领域:
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
of rudimentary homolog (Drosophila) (ERH), mRNA 1028 NM_004048 Homo sapiens beta-2-microglobulin (B2M), mRNA 4992 NM_000239 Homo sapiens lysozyme (renal amyloidosis) (LYZ), mRNA 796 NM_000269 Homo sapiens non-metastatic cells 1, protein (NM23A) expressed
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
3 分线外挣扎的你,看别人如何登顶 Nature、Science
),杯状细胞(G),基底细胞和纤毛状细胞(C)。第二幕 ASL 中抗菌物质的数量不是元凶一、是否为 ASL 抗菌物质的表达,转录和翻译差异?实验 1. 转录产物,分别用基因芯片和定量 RT-PCR 检测,表达没差异 (Table S1, Fig. S1)实验 2. 宿主防御相关蛋白基因,表达没差异 (Table S2)实验 3. 乙酰甲胆碱刺激产生的 ASL,含量最丰富的抗菌物质,lysozyme(溶菌酶)和 lactoferrin(乳糖肝褐质),PLUNC 和 SP-A,没差异 (Fig. 2a)。二
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