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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Beta-enolase (ENO3), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Beta-enolase (ENO3), partial品名简称:
Recombinant Human ENO3 protein, partial货号:
CSB-RP131674h规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ENO3Uniprot No.:
P13929别名:
2-phospho-D-glycerate hydro-lyase;Enolase 3Muscle-specific enolase ;MSESkeletal muscle enolase种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
50.1 kDa表达区域:
7-432aa氨基酸序列:
FAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPK蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Appears to have a function in striated muscle development and regeneration.功能2:
Appears to have a function in striated muscle development and regeneration.文献:
Structural features of the human gene for muscle-specific enolase. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA.Giallongo A., Venturella S., Oliva D., Barbieri G., Rubino P., Feo S.Eur. J. Biochem. 214:367-374(1993)研究领域:
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文献和实验Identification, Characterization, and Differentiation of Human Prostate Cells
cells as they express neuroendocrine markers, such as chromagranin A and neurone-specific enolase (16 ,17 ). The smooth muscle cells, fibroblasts, and the endothelial cells in the blood vessels of prostate stroma can be distinguished from one
enolase 非神经元性烯醇化酶 NP neuropeptide 神经肽 NPY neuropeptide Y 神经肽Y NSE neuron-specific enolase 神经元特异性烯醇化酶 NT neurotensin 神经降压肽 OD optical density 光密度 6-OHDA 6-hydroxydopamine 6-羟多巴胺 ORF open reading frame 开放读框 ORT optimum
Survey of Protein Engineering Strategies
. Vick J.E., Schmidt, D.M.Z., and Gerlt, J.A. 2005. Evolutionary potential of (beta/alpha)8‐barrels: In vitro enhancement of a “new” reaction in the enolase superfamily. Biochemistry 44:11,722‐11,729
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