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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 (NDUFS5)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 (NDUFS5)品名简称:
Recombinant Human NDUFS5 protein货号:
CSB-RP027354h规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
NDUFS5Uniprot No.:
O43920别名:
Complex I-15KDA ;CI-15KDANADH-ubiquinone oxidoreductase 15KDA subunit种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
39.4 kDa表达区域:
2-106aa氨基酸序列:
PFLDIQKRFGLNIDRWLTIQSGEQPYKMAGRCHAFEKEWIECAHGIGYTRAEKECKIEYDDFVECLLRQKTMRRAGTIRKQRDKLIKEGKYTPPPHHIGKGEPRP蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Accessory subunit of the mitochondrial mbrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.功能2:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.文献:
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F., Ghosh S.S., Capaldi R.A.J. Biol. Chem. 278:13619-13622(2003)研究领域:
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens ribosome binding protein 1 homolog 180kDa (dog) (RRBP1), mRNA 658 NM_004552 Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase) (NDUFS5), mRNA 936 NM_004450 Homo sapiens enhancer
。 ( 2 ) 为了得到高浓度的特异重组蛋白提取物(如在低拷贝质粒上重组蛋白质)用于随后的蛋白质纯化,我们可以同时用 100 μl 裂解液分别裂解两个相同的 1 ml 培养物的沉淀物,合并得到 200 μl 裂解物,供下一步分析 [15]。 ( 3 ) 小心吸取上清液,只将那些澄清的上清液转移到过滤板上,以确保在随后的过滤中板不会被堵塞。 ( 4 ) 使用适用于微孔板的真空泵。 ( 5 ) 确保在过滤后没有液体残留在过滤板下面;如果有的话,必须手动清除。为了避免这个问题的出现,过滤要在足够的真空
Metallomics Using Inductively Coupled Plasma Mass Spectrometry
Basic Protocol 1: ICP‐MS Analysis of Soluble Protein Samples Basic Protocol 2: Preparation of Washed Cytoplasmic Extract for the Identification of Soluble Metal‐Macromolecular Complexes (≥3 kDa) Basic Protocol
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