- 询价
- Agrisera
- AS03 037A
- 瑞典
- 2026年03月07日
- Western blot (WB)
- Rabbit
- Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, C
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
RbcL | Rubisco large subunit, form I (affinity purified)
- 抗体英文名：
RbcL | Rubisco large subunit, form I (affinity purified)
- 靶点：
This antibody is especially suitable for quantifying of Rubisco in plant and algal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologo
- 应用范围：
Western blot (WB)
- 宿主：
Rabbit
- 适应物种：
Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, C
- 抗原来源：
O03042 , P05698 , P0C510 , P00877
- 级别：
Affinity purified serum
- 供应商：
Agrisera AB
- 克隆性：
单克隆
- 保存条件：
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the ca
- 形态：
Lyophilized in PBS pH 7.4.
- 免疫原：
KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana O03042, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardti
- 规格：
50 µg
application example
Total protein from Populus T89 were extracted with “KEB buffer”, precipitated with ethanol on ice and denatured with “loading buffer” at 100°C for 10 min, separated on 8% SDS-PAGE and blotted O/N to PVDF using (wet blot) tank transfer. Blots were blocked with 5%TBS milk, for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 TBS for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly with TBS-T, then washed for 1h in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP-conjugated, from Agrisera, AS09 602) diluted to 1:5000 in TBS-M (milk 5%) for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, for 10s increment until exposure time of 30s total.
Courtesy Dr. Mark Ruhl, Umeå Plant Science Centre, Sweden
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文献和实验the Drosophila holoenzyme form to near homogeneity for the first time from any source (4 ). The use of partially purified mitochondria was also critical, because in this and subsequent purification schemes for other mitochondrial proteins, we have observed
Purifi cation of Multiprotein Histone Acetyltransferase Complexes
with other proteins to form stable multisubunit complexes. Importantly, these peripheral proteins significantly influence the functions of the catalytic HAT subunit by regulating its intrinsic catalytic activity and/or by modulating its target substrate selectivity
are separated and purified by reverse-phase HPLC or thin-layer chromatography (TLC) (Fig. 14). The isolated peptides are then cross-linked via their C termini to an inertmembrane (e.g. Immobilon P; PerSeptive Biosystems). The radioactive membrane is subjected
