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AtPCaP1 | Arabidopsis thaliana

plasma membrane cation-binding protein-1
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  • 询价
  • Agrisera
  • AS10 931
  • 瑞典
  • 2025年11月17日
  • Western blot (WB)
  • Rabbit
  • Arabidopsis thaliana, Raphanus sativus (41 kDa), Brassica rapa (42 kDa), Brassica rapa var. glabra Regel (43 kDa), Brassica oleracea var. italica (41 kDa)
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1

    • 抗体英文名

      AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1

    • 靶点

      AtPCaP1 is a protein associated with plasma membrane with ability to bind Ca, calmodulin and phosphatidylinositol phoshphates. Alternative names: At4g20260, endomembrane-associated protein.

    • 应用范围

      Western blot (WB)

    • 宿主

      Rabbit

    • 适应物种

      Arabidopsis thaliana, Raphanus sativus (41 kDa), Brassica rapa (42 kDa), Brassica rapa var. glabra Regel (43 kDa), Brassica oleracea var. italica (41 kDa)

    • 抗原来源

      Q96262

    • 级别

      Serum

    • 供应商

      Agrisera AB

    • 克隆性

      单克隆

    • 保存条件

      Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the ca

    • 形态

      Lyophilized

    • 免疫原

      KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence UniProt: Q96262, TAIR: At4g20260

    • 规格

      100 µl

    It is confirmed that the nock-out mutant (SALK_022955, Col-0 background) has no mRNA or protein of PCaP1. In the immunoblot, crude membrane fractions prepared from wild-type and pcap1 knock-out mutant plants were examined. Therefore the antibody to PCaP1 specifically recognizes PCaP1 and can be used for immunoblotting. For application in immunocytochemistry cross reacting band in proximity of Rubisco has to be removed by incubation of an antibody with a part of memrane with background band. Protocol for preparation of crude membranes This protocol will help to prepare crude membrane fractions from very small amount of plant tissue. (1) Homogenize roots or root tips with the buffer without SDS.(2) Centrifuge the homogenate in Eppendorf tubes at 2 000 rpm for 2 min to remove cell wall, nuclei, plastids and mitochondria. (3) Centrifuge the obtained supernatant at 30 000 g for 20 min to separate soluble components. Please use adequate tubes for ultracentrifugation. (4) Suspend the obtained precipitate (crude membranes) with SDS buffer with DTT or beta-mercaptoethanol. (5) Heat the sample solution obtained the step (4) at 70 C for 5 min. (6) Apply the denatured sample to SDS-PAGE.Courtesy Prof. Msayoshi Maeshima, Nagoya University, Japan

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