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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Grancalcin (GCA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Grancalcin (GCA)品名简称:
Recombinant Human GCA protein货号:
CSB-EP009307HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GCAUniprot No.:
P28676别名:
GCA; GCL; Grancalcin种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
51 kDa表达区域:
1-217aa氨基酸序列:
MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.功能2:
Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.文献:
"Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner." Lollike K., Johnsen A.H., Durussel I., Borregaard N., Cox J.A. J. Biol. Chem. 276:17762-17769(2001)研究领域:
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文献和实验请教高手,我做一蛋白的定点突变,采用overlap的方式,但当我把短片段扩出来后,就怎么也不能将全片段(1.58Kb)扩出来,我不知道是不是引物的问题,请大家帮我看看 这是整个CD序列
Wnt/β-catenin信号通路在实验性大鼠肝癌形成过程中的作用
Wnt /β-catenin信号转导通路是一条在生物进化中极为保守的通路。在正常的体细胞中,β-catenin只是作为一种细胞骨架蛋白在胞膜处与E-cadherin形成复合体对维持同型细胞的黏附、防止细胞的移动发挥作用。只有当细胞外Wnt信号分子与细胞膜上特异性受体Frizzled蛋白结合激活胞内的d ishevelled(散乱的)蛋白导致GSK3B失活,使α-catenin避免被磷酸化而遭降解的命运 , β-catenin才能在胞质中积累起来。当胞质中B-catenin的浓度达到一定水平
29.0 Gly GGT 26.0 GCC 16.5 GGC 8.0 GCA 15.0 GGA 19.1 GCG 3.8 GGG 5.8 表 2 最高频率密码
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