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Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, Biotin conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified mouse IgG, whole molecule
- 形态:
Lyophilized
- 保存条件:
Store lyophilized material at 2-8°C.For long time
- 克隆性:
Polyclonal
- 适应物种:
Reacts with: heavy (γ) chains chains on mouse IgG light chains on all on all mouse immunoglobulins
- 级别:
分子生物学级
- 供应商:
Agrisera
- 宿主:
Llama
- 应用范围:
The optimal working dilution should be determined by the investigator.
- 靶点:
Llama anti-Mouse IgG (H&L), HRP Conjugated is a secondary antibody which binds to heavy (γ) chains chains on mouse IgG light chains on all mouse immunoglobulins
- 抗体名:
Llama anti-Mouse IgG (H&L), HRP Conjugated
- 规格:
1 mg
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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