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DMPO | DMPO nitrone adduct (cl

one N1664A)
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  • 询价
  • Agrisera
  • 瑞典
  • AS11 1632
  • 2025年11月18日
  • 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)
  • Mouse
  • DMPO (species independent)
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin

    • 亚型

      IgG1

    • 形态

      Liquid

    • 保存条件

      Store at -20°C for one year. once reconstituted ma

    • 克隆性

      Monoclonal

    • 标记物

      Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.

    • 适应物种

      DMPO (species independent)

    • 级别

      分子生物学级

    • 供应商

      Agrisera

    • 宿主

      Mouse

    • 应用范围

      1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)

    • 靶点

      DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA ra

    • 抗体英文名

      Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.

    • 抗体名

      DMPO | DMPO nitrone adduct (clone N1664A)

    • 规格

      100 µl

    DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

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    图标文献和实验
    相关实验
    • EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer Syste

      with 5-diethoxylphosphoryl-5-methyl-1-pyrroline N -oxide (DEPMPO), indicating that FMN/FMN-binding domain (complex I), ubiquinone (complex I and III), FAD/FAD-binding domain (complex II), and cytochrome b (complex III) control the mediation of O2 •– production

    • Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS

      by spin trapping and ESR procedures often has been done using 5,5-dimethylpyrroline N-oxide (DMPO) as the spin trap (5 –9 ). This spin trap very rapidly reacts with OH to produce a stable free radical adduct having a characteristic ESR spectrum (5 –9 ).

    • 超氧化物歧化酶(SOD)的检测方法

      自旋共振(ESR)光谱法 在室温下,溶液中O2-的ESR信号不能直接被检测出,但能够通过自旋捕捉法间接获得。最常见的诱捕剂是5,5-dimethyl-1-pyrroline N-oxide(DMPO)。由于O2- 诱捕DMPO会产生一个特殊的光谱图,因此ESR法是O2-检测的方法中特异性最好的一种。然而,DMPO与O2-间的二阶速度常数比O2-自发反应常数相对要低,因此需要向溶液中加入大量的DMPO(如:终浓度为0.45M)。这样,每次试验所要用的DMPO将花去大笔的经费。 这种方法的另一

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