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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Kallikrein-2 (KLK2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Kallikrein-2 (KLK2)品名简称:
Recombinant Human KLK2 protein货号:
CSB-EP012453HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
KLK2Uniprot No.:
P20151别名:
Glandular kallikrein-1种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
30.2 kDa表达区域:
25-261aa氨基酸序列:
IVGGWECEKHSQPWQVAVYSHGWAHCGGVLVHPQWVLTAAHCLKKNSQVWLGRHNLFEPEDTGQRVPVSHSFPHPLYNMSLLKHQSLRPDEDSSHDLMLLRLSEPAKITDVVKVLGLPTQEPALGTTCYASGWGSIEPEEFLRPRSLQCVSLHLLSNDMCARAYSEKVTEFMLCAGLWTGGKDTCGGDSGGPLVCNGVLQGITSWGPEPCALPEKPAVYTKVVHYRKWIKDTIAANP蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.功能2:
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.文献:
"Primary structure of a human glandular kallikrein gene." Schedlich L.J., Bennetts B.H., Morris B.J. DNA 6:429-437(1987)研究领域:
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文献和实验Evaluation of Peptides as Protease Inhibitors and Stimulators
. We present here detailed protocols for basic evaluation of the effects of peptides on the activity of proteases, using kallikrein-related peptidases KLK2 and KLK3 (also known as hK2 and PSA), and trypsin as examples. KLK2 and KLK3 are major prostatic
现的即时反应。( 2)以舒缓激肽( bradykinin)、赖氨酰舒缓激肽( kallidin)、甲硫氨酰 -赖氨酰 -舒缓激肽( methio- nyl- lysyl- bradykinin)为代表的多肽类。其共同的特征是可使血管透性亢进、平滑肌收缩、血管扩张,促进白细胞游走。舒缓激肽和赖氨酰舒缓激肽的结构已被确定。( 3)血纤维溶解酶( plasmin)、激肽释放酶( kallikrein)、球蛋白透性因子( globulin- PF)等蛋白酶( protease),其本身并不能成为血管透性的作用
作用 (1)激肽系统:组织损伤首先激活血浆酶原Hageman因子(又称凝血因子Ⅻ),引起凝血酶级联反应。反应过程中首先是激肽释放酶(kallikrein)活化,然后产生炎症介质舒缓激肽(bradykinin)。这是激肽系统中一类血管活性碱性肽,可增加血管通透性,引起疼痛和平滑肌收缩。舒缓激肽(kinin)还可裂解C5,产生的C5a使肥大细胞释放炎症介质。 (2)凝血系统:始于血管损伤大量产生的凝血酶。凝血酶作用于血浆和组织液中的血纤蛋白原,产生血纤蛋白(fibrin)和血纤肽
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