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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Glutathione S-transferase P 1 (Gstp1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14844.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3456.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
Gstp1Uniprot No.:
P19157别名:
GST YF-YFGST class-piGST-piBPreadipocyte growth factor种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
25.5 kDa表达区域:
2-210aa氨基酸序列:
PPYTIVYFPVRGRCEAMRMLLADQGQSWKEEVVTIDTWMQGLLKPTCLYGQLPKFEDGDLTLYQSNAILRHLGRSLGLYGKNQREAAQMDMVNDGVEDLRGKYVTLIYTNYENGKNDYVKALPGHLKPFETLLSQNQGGKAFIVGDQISFADYNLLDLLLIHQVLAPGCLDNFPLLSAYVARLSARPKIKAFLSSPEHVNRPINGNGKQ蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Can metabolize 1-chloro-2,4-dinitrobenzene. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration .内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.Mol. Cell 50:919-930(2013)Function:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Can metabolize 1-chloro-2,4-dinitrobenzene. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration (By similarity).风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Preparation of Glutathione-S-Transferase (GST) Fusion Proteins Margret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia, PA 19111 Jason R. Orlinck
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
-19]; xChIP:用来研究DNA及低结合力蛋白,采用甲醛或紫外线进行 DNA 和蛋白交联,超声波片段化染色质,然后进行片段富集及后续分析,适用于多数非组蛋白的蛋白,例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后,对 DNA 进行 PCR 扩增,验证目标序列的存在。除验证实验外,ChIP DNA也可以进行测序分析,这种方法被称为 ChIP-seq [22] ;也可做芯片分析,这种方法被称为 ChIP on CHIP 或 ChIP
Affinity Purification of Proteins Binding to GST Fusion Proteins
the use of proteins fused to glutathione?S ?transferase (GST fusion proteins) to affinity purify other proteins, a technique also known as GST pulldown purification. The describes a strategy in which a GST fusion protein is bound to agarose affinity beads
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