NCL|NCL蛋白|NCL人蛋白|NCL Human Pro

Expressed Protein Ligation for Protein Semisynthesis and Engineering

for NCL aside from the rapid isolation of highly pure recombinant proteins. Expressed protein ligation extends the scope of NCL by overcoming the size limitation of target proteins accessible to synthesis. The intein splicing and EPL

Preparation of C-terminally Modified Chemokines by Expressed Protein Ligation

. In the present chapter we mainly focus on the fabulous history of native chemical ligation (NCL) and provide a general protocol for the preparation of C-terminally modified SDF-1α including tips and tricks for practical work. We believe that this protocol

Semi-synthesis of Glycoproteins from E. coliThrough Native Chemical Ligation

Sufficient quantities of homogeneous samples of post-translationally modified proteins are often not readily available from biological sources to facilitate structure–function investigations. Native chemical ligation (NCL) is a convenient