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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Gamma-crystallin B (Crygb)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Gamma-crystallin B (Crygb)品名简称:
Recombinant Rat Crygb protein货号:
CSB-YP006018RA规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CrygbUniprot No.:
P10066别名:
Gamma-B-crystallin Gamma-crystallin 1-2种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
23 kDa表达区域:
2-175aa氨基酸序列:
GKITFFEDRGFQGRCYECSSDCPNLQTYFSRCNSVRVDSGCWMLYERPNYQGHQYFLRRGDYPDYQQWMGFSDSIRSCRLIPQHSGTYRMRIYERDDFRGQMSEITDDCLSLQDRFHLSEIHSLNVMEGCWVLYEMPSYRGRQYLLRPGEYRRYLDWGAANAKVGSFRRVMDFY蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Crystallins are the dominant structural components of the vertebrate eye lens.功能2:
Crystallins are the dominant structural components of the vertebrate eye lens.文献:
"Nucleotide sequence of the rat gamma-crystallin gene region and comparison with an orthologous human region." den Dunnen J.T., van Neck J.W., Cremers F.P.M., Lubsen N.H., Schoenmakers J.G.G. Gene 78:201-213(1989)研究领域:
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文献和实验(3000rpm),20min,充上清,以x ml生理盐水溶解沉淀,再逐滴加饱和硫酸铵x/2ml。 ↓置4℃3h以上,[此时,(NH4)2SO4的饱和度为33%] 重复上述第二步过程1~2次。末次离心后所得沉淀物为γ-球蛋白,以0.02% mol/L pH7.4PBS溶解至x ml装入透析袋。 ↓对PBS充分透析、换液3次,至萘氏 试剂 测透析外液无黄色,即无NH4+为止。
beta-actin B-actin GAPDH(Human, rat and mouse) GAPDH(人/小鼠/大鼠 通用) GAPDH GAPDH/ NM_002046 GAPDH(大鼠、小鼠、人) β-actin(大鼠、小鼠、人) beta-actin beta-actin PCR or RT-PCR EBV BamHⅠ-W EBV Qp EBV Wp EBV Cp EBV Fp EBV LMP1 EBV LMP2A EBV
James Hardwick angiotensin assay protocol
This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviously use fewer or more cells, depending on your needs. Don't skimp
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