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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Guanidinoacetate N-methyltransferase (Gamt)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Guanidinoacetate N-methyltransferase (Gamt)品名简称:
Recombinant Rat Gamt protein货号:
CSB-EP009227RA规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GamtUniprot No.:
P10868别名:
Gamt; Guanidinoacetate N-methyltransferase; EC 2.1.1.2种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length来源:
E.coli分子量:
30.4 kDa表达区域:
1-236aa氨基酸序列:
MSSSAASPLFAPGEDCGPAWRAAPAAYDTSDTHLQILGKPVMERWETPYMHSLAAAAASRGGRVLEVGFGMAIAASRVQQAPIKEHWIIECNDGVFQRLQNWALKQPHKVVPLKGLWEEEAPTLPDGHFDGILYDTYPLSEETWHTHQFNFIKTHAFRLLKPGGILTYCNLTSWGELMKSKYTDITAMFEETQVPALLEAGFQRENICTEVMALVPPADCRYYAFPQMITPLVTKH蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.文献:
crystal structures of guanidinoacetate methyltransferase ternary complexes.Komoto J., Yamada T., Takata Y., Konishi K., Ogawa H., Gomi T., Fujioka M., Takusagawa F.Biochemistry 43:14385-14394(2004)研究领域:
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文献和实验【信息】Northstar BioProducts(NSBP)
苷酶)、Glycopeptidase(糖肽酶)、Pullulanase(普鲁兰多糖酶)、Endopeptidase(肽链内切酶)、PNGase F(N-糖酰胺酶F) 编号 包装 100095-1 N-Acetylmuramidase SG, (Streptomyces globisporus), EC 3.2.1.17 10 mg Peptidoglycan N-acetylmuramoylhydrolase (Muramidase, M-1 enzyme) Lyophilized powder
2.4.1.31.)、纤维糊精磷酸化酶(EC2.4.1.49.)、1,3-β-D-葡聚糖磷酸化酶(EC2.4.1.97.)、嘧啶核苷磷酸化酶(EC2.4.2.2.)、尿苷磷酸化酶(EC2.4.2.3.)、胸腺嘧啶磷酸化酶(EC2.4.2.4.)、鸟苷磷酸化酶(EC2.4.2.15.)等。例如最有代表性的磷酸化酶是糖原磷酸化酶,糖原在体内降解过程中,该酶是催化糖原还原性末端葡萄糖残基的d-1,4-糖苷键断裂,反应,生成1-磷酸葡萄糖和少了一个葡萄糖基的糖原分子(见图)。这类酶多数是作为生化试剂应用
标准化处理。对于各种药物,在计算时需减去只含药物溶剂时对照细胞表现出的背景值。小分子药物相应溶剂为DMSO,多肽药物相应溶剂为0.1% 的BSA 。 将检测结果数据导出至Microsoft Excel,进行后续计算。确定每孔细胞的最大与最小细胞指数值,计算重复检测(n=2)的均值标准差与平均值。对于对照孔细胞来说,对重复检测 8 个孔的均值标准差进行测定。将这些数值平均并乘以 3 以计算“hit”阈值。对于 Z 因子与 EC50 计算来说,使用RTCA MP提供的动态反应曲线,选择一时间点作为细胞系及药物综合
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