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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Ketohexokinase (Khk)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Ketohexokinase (Khk)品名简称:
Recombinant Mouse Khk protein货号:
CSB-EP012157MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
KhkUniprot No.:
P97328别名:
Hepatic fructokinase种属:
Mus musculus (Mouse)蛋白长度:
Full Length来源:
E.coli分子量:
36.4 kDa表达区域:
1-298aa氨基酸序列:
MEEKQILCVGLVVLDIINVVDKYPEEDTDRRCLSQRWQRGGNASNSCTVLSLLGARCAFMGSLAPGHVADFLVADFRQRGVDVSQVTWQSQGDTPCSCCIVNNSNGSRTIILYDTNLPDVSAKDFEKVDLTRFKWIHIEGRNASEQVKMLQRIEEHNAKQPLPQKVRVSVEIEKPREELFQLFSYGEVVFVSKDVAKHLGFQPAVEALRGLYSRVKKGATLVCAWAEEGADALGPDGQLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSKGNSMQEALRFGCQVAGKKCGLQGFDGIV蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.功能2:
/文献:
"Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling." Kislinger T., Cox B., Kannan A., Chung C., Hu P., Ignatchenko A., Scott M.S., Gramolini A.O., Morris Q., Hallett M.T., Rossant J., Hughes T.R., Frey B., Emili A. Cell 125:173-186(2006)研究领域:
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文献和实验Cell Metab:浙大吕志民团队揭示肿瘤细胞 Warburg 效应促进肿瘤免疫逃逸
2 从线粒体外膜脱落,进入细胞浆中。在细胞浆中,HK2 结合 NF-κB 的抑制因子 IκBα。 重要的是,HK2 发挥了不依赖于其经典代谢功能的新功能,即作为一个蛋白激酶磷酸化 IκBα 的 Thr 291 位点。该磷酸化促进了蛋白酶 μ-calpain 与 IκBα 的结合并进一步降解 IκBα,进而使转录因子 NF-κB 入核,最终促进了 PD-L1 的表达并导致了肿瘤的免疫逃逸。作者还发现,使用己糖激酶的抑制剂与 PD-1 抗体联用治疗小鼠胶质瘤,可以显著提升 PD-1 抗体的治疗
果糖才是肥胖的真凶?Nature 最新文章揭示高果糖饮食如何
,丙酮酸与磷酸烯醇丙酮酸(PEP)的比例显著降低,与丙酮酸激酶的抑制效果一致。此外,PKM2 在缺氧组织中的表达很高,例如肿瘤和肠绒毛等。图片来源:Nature果糖主要由糖转运蛋白 GLUT5 转运到肠细胞中,然后被 KHK 磷酸化形成 1-磷酸果糖(F1P)。由于 F1P 在结构上类似于 PKM2 的内源性调节因子 1,6-二磷酸果糖(FBP),因此作者假设 F1P 可能直接抑制 PKM2 的活性。研究发现,F1P 会与 FBP 竞争结合 PKM2 的同一位点,而且一旦结合就能够直接抑制 PKM
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AMPK signaling and mitochondrial respiration。 该研究发现果糖代谢通路中的多个调节因子,果糖转运蛋白 SLC2A5 和果糖代谢酶己酮糖磷酸激酶 (KHK),在肝细胞癌 (HCC) 肿瘤内皮细胞中表达上调,饮食果糖增强了肿瘤血管生成、肿瘤生长和转移。 图 9:来源 Cancer Research 10. Science Advances:揭示细胞有丝分裂期转录调控动态机制 目前,细胞有丝分裂期转录调控动态机制不明。 2023 年 1 月 25
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