Wnk1|Wnk1蛋白|Wnk1小鼠蛋白|Wnk1 Mous

ERK5 MAPK信号通路图

both of which are localized to the C-terminal portion of the protein that also accounts for its more than twice the size of the other MAPKs. Adapters such as Sh2d2a (known as Lad or Ribp), upstream kinases such as Wnk1 or activators including members of the Ras family

Protein Therapeutics: Mouse, Humanized, and Human Antibodies

Antibodies play a vital role in immune defense against pathogens. They are globular glycoproteins produced by plasma cells in response to the antigenic stimulation of B-lymphocytes. Their circulation in blood and lymph contributes to the humoral

【原创】Protein A Sepharose 之进化篇。。。

洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下