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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human DNA polymerase theta (POLQ), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3468.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
POLQUniprot No.:
O75417别名:
DNA polymerase eta种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
33.3 kDa表达区域:
321-554aa氨基酸序列:
GDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYA蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
Epigenetics and Nuclear SignalingReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验The Polymerase Chain Reaction (PCR)
a means of selectively amplifying a particular segment of DNA. • The segment may represent a small part of a large and complex mixture of DNAs:e.g. a specific exon of a human gene. • It can be thought of as a molecular photocopier. A Molecular
We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template
能催化从四种5′-脱氧核苷三磷酸游离出焦磷酸而使DNA进行聚合反应的酶。EC2.7.7.7.。必须以DNA为模板,合成的DNA与模板具有互补的碱基排列(碱基互补性)。是与DNA复制和修复有关的重要的酶,虽可从许多组织分离出来,但对大肠杆菌的这种酶的研究较为先进。康伯格(A.Kornberg,1958)最初从大肠杆菌将此酶纯化,并弄清了其结构和特异性,但以后经过分子遗传学的研究,已明确了这种酶主要与DNA修复有关。因此重新进行与DNA复制有关酶的检索,发现两种新的酶。康伯格最初发现的酶称为
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