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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Isocitrate dehydrogenase [NADP] Cytoplasmic domain (Idh1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Isocitrate dehydrogenase [NADP] Cytoplasmic domain (Idh1), partial品名简称:
Recombinant Mouse Idh1 protein, partial货号:
CSB-EP010989MO规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Idh1Uniprot No.:
O88844别名:
Cytosolic NADP-isocitrate dehydrogenaseIDPNADP(+)-specific ICDHOxalosuccinate decarboxylase种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
E.coli分子量:
49.2 kDa表达区域:
7-407aa氨基酸序列:
GGSVVEMQGDEMTRIIWELIKEKLILPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKYNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPKDGTQKVTYMVHDFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKKYKSQFEAQKICYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLICPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWSRGLAHRAKLDNNTELSFFAKALEDVCIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKA蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013)研究领域:
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文献和实验Glucose Dehydrogenase for the Regeneration of NADPH and NADH
their applicability we coupled an NADP+ -dependent, (R)-specific alcohol dehydrogenase (ADH) from Lactobacillus kefir with the glucose dehydrogenase from B. subtilis. The ADH reduces prochiral ketones stereoselectively to chiral alcohols. The reduction requires NADPH
),催化基本反应为: RH+O2+NADPH+H+→ROH+NADP++H2O 单加氧酶能直接激活氧分子,使其中一个氧原子直接加入底物分子中形成羟化物或环氧化物,另一个氧原子则被NADPH还原为水。由于一个氧分子发挥了两种功能,故单加氧酶系又称为混合功能氧化酶;又因底物的氧化产物是羟化物,所以又称为羟化酶。加单氧酶的(见图1) 图1 加单氧酶的反应 肝细胞微粒体内存在的还原酶主要有硝基还原酶和偶氮还原酶,是第Ⅰ相反应的主要还原酶,能使硝基化合物和偶氮化合物还原生成胺类(见图
Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO
Nitric oxide synthase (NOS) catalyzes the conversion of L -arginine, molecular oxygen, and nicotinamide adenine dinucleotide phosphate (NADPH) to NO, citrulline, and NADP+ (reviewed in ref. 1 ). This chapter describes the measurement of NOS
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