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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见说明书
- 保质期：
见说明书
- 英文名：
Recombinant Human Kinetochore protein NDC80 homolog(NDC80)
- 库存：
99
- 供应商：
武汉华美生物/CUSABIO
- 规格：
20ug/100ug/1mg
NDC80蛋白|Recombinant Human Kinetochore protein NDC80 homolog(NDC80)|大肠杆菌/毕赤酵母/哺乳动物细胞/杆状病毒-昆虫细胞/无细胞 五大表达系统
Uniprot ID：O14777
别名：from Entrez Gene ,; HEC; Highly expressed in cancer; Highly expressed in cancer protein; Highly expressed in cancer rich in leucine heptad repeats; HsHec1; hsNDC80; Kinetochore associated 2; Kinetochore associated protein 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC2; ndc80; NDC80 homolog kinetochore complex component; NDC80 kinetochore complex component homolog; NDC80, S. cerevisiae, homolog of; NDC80_人; Retinoblastoma associated protein HEC; Retinoblastoma-associated protein HEC; TID3
种属：Homo sapiens (Human)
氨基酸序列：参见官网
微信ID: Cusabio01 电话： 027-87939808
详情请登录：www.cusabio.cn
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文献和实验Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs
The identification of protein binding partners often facilitates understanding of protein complex function. However, identifying binding partners has proven difficult because proteins are often bound to insoluble structures
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下
Purification of Human Complement Protein C5
Complement C5 is cleaved by proteolysis in the terminal phase of complement activation generating the pro-inflammatory C5a and membrane attack complex nucleator C5b. Whereas purification of its paralogues C3 and C4 from plasma is relatively
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