PTGS2|PTGS2蛋白|PTGS2兔蛋白|PTGS2 R

Antisense Inhibition of Protein Synthesis and Function: Rabbit Retinal Protein

When considering the use of antisense technology for in vivo apphcatron, whether for therapeutic development or for the creation of animal models for human diseases, a major problem is that most extracellular compartments are constantly

Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein

The rabbit reticulocyte lysate (RRL) is a convenient system for in vitro translation of transcription factors (1 ). By using in vitro-synthesized transcription factors, the time-consuming and often difficult process of protein purification

【原创】Protein A Sepharose 之进化篇。。。

洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下