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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Isthmin-1 (Ism1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Isthmin-1 (Ism1)品名简称:
Recombinant Mouse Ism1 protein货号:
CSB-YP011850MO规格:
20μg价格:
2208LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Ism1Uniprot No.:
A2ATD1别名:
Ism种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
50.1 kDa表达区域:
30-454aa氨基酸序列:
SDRQDAAAGNNNLNLESDSTSETSFPLSKEAPEEHQVVHQPFPRQRFPPETGHPSLQRDGPRSFLLDLPNFPDLSKADINGQNPNIQVTIEVVDGPDSEAEKDQHPENKPSWSLPAPDWRAWWQRSLSLARTNSGDQDDKYDSTSDDSNFLSVPRGWDRPAPGHRTFETKEQPEYDSTDGEGDWSLWSVCSVTCGNGNQKRTRSCGYACIATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEEFNATKLFEVDMDSCERWMSCKSEFLKKYMHKVINDLPSCPCSYPTEVAYSTADIFDRIKRKDFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDVLPWIICKGDWSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Acts as an angiogenesis inhibitor.功能2:
Acts as an angiogenesis inhibitor.文献:
"Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain-hindbrain organizer." Pera E.M., Kim J.I., Martinez S.L., Brechner M., Li S.-Y., Wessely O., De Robertis E.M. Mech. Dev. 116:169-172(2002)研究领域:
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文献和实验「铲屎官」注意了!剑桥大学揭示这些基因的改变或让猫猫狗狗成为
细胞 iBMDM 和犬的巨噬样细胞 DH82。结果表明小鼠巨噬细胞感染 2 小时后开始裂解,而犬的巨噬样细胞经过 12 小时仍存活,表明犬的细胞对于鼠伤寒沙门菌的耐受性更高。接着,研究者在小鼠模型上通过 CRISPR-Cas9 技术构建了与 caspase-1/-4 融合蛋白催化作用相当的重组蛋白。小鼠细胞在脂多糖(LPS)刺激下很快裂解,而犬的巨噬样细胞对于 LPS 的刺激则无反应。进一步的体外实验表明,caspase-1/-4 融合蛋白能激活 IL-1β 并且同时切割消皮素 D。图片来源:Cell
,并且 EST12-RACK1 诱导的细胞焦亡在 M.tb 诱导的免疫中起关键作用。 研究内容: 1、RD 区编码蛋白筛选:对 40 种 RD 区(RD1-3、11-14)重组蛋白进行筛选(巨噬细胞焦磷酸相关蛋白),通过 LDH 释放实验细胞毒性分析,发现 EST12 对巨噬细胞有显著焦解作用。 2、菌株构建:构建 M. tb H37Rv ΔEST12(Rv1579c 敲除菌株)、BCG-EST12(Rv1579c 过表达菌株)、M. smeg–EST12(Rv1579c 过表达菌株)。 3、小鼠
域 / 折叠花样的总数是有限的。蛋白结构的模块化本质允许一级序列存在插入或删除。一般来说,结构域与功能一一对应(如 Kinase),但并不总是如此(如 TIM 桶),类似的结构可具有不同的功能 , 不同的结构可具有相似的功能。蛋白质结构与功能研究的技术路线如下:对整个技术路线,我们挑选其中的几个来讲一讲。首先就是蛋白结晶片段的选取,前面讲过,一般来说,蛋白质结构域与功能一一对应。因此,为了降低整个项目的难度(多个结构域会增加蛋白制备难度和结晶难度),通常会按照结构域选取蛋白质片段进行重组蛋白制备及结晶
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