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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Apolipoprotein B-100 (APOB), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥10872.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2514.0 |
| 规格: | 20μg | 产品价格: | ¥1344.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
APOBUniprot No.:
P04114别名:
Apo B-100种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
27.2 kDa表达区域:
28-127aa氨基酸序列:
EEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMS蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Apolipoprotein B is a major protein constituent of chylomicrons, LDL and VLDL. Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Epigenetics and Nuclear SignalingReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Human liver apolipoprotein B-100 cDNA: complete nucleic acid and derived amino acid sequence." Law S.W., Grant S.M., Higuchi K., Hospattankar A.V., Lackner K.J., Lee N., Brewer H.B. Jr. Proc. Natl. Acad. Sci. U.S.A. 83:8142-8146(1986)Function:
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文献和实验人的载脂蛋白有两种表达形式: apoB-48和apoB-100。该基因的序列在肝、肠组织细胞中是相同的,但产物不同。在肝细胞中,产物为4563个氨基酸残基的肽:apoB-100; 但在肠细胞中,产物为只含2152个氨基酸残基的apoB-48, 缺失了apoB-100的N端同LDL受体结合的结构域。原因是在mRNA水平上将2153位的谷氨酰胺的密码子CAA中的C编辑成为U,形成一个终止密码子UAA,结果使蛋白质的合成提前终止,合成一个新的相对分子质量约为250kDa的蛋白
作者:李琳,杨镍,何广源,全红兵 [摘 要] 目的:对中生北控生物科技股份有限公司生产的载脂蛋白AI、B液体双试剂的性能进行评价。方法:采用中生载脂蛋白AI、B液体双试剂,用免疫透射比浊法测定质控血清和住院病人血清载脂蛋白AI(ApoAI)及载脂蛋白B(ApoB),分析中生载脂蛋白AI、B液体双试剂的精密度与稳定性、准确度、性线,并与英国Randox试剂作对照。结果:中生的载脂蛋白AI、B液体试剂测定均值偏差小于2%;对高、低
gene encoding apolipoprotein B (apoB) after intravenous injection in mice. Administration of chemically modified siRNAs resulted in silencing of the apoB messenger RNA in liver and jejunum, decreased plasma levels of apoB protein, and reduced total
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