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APOB/APOB蛋白Recombinant Human A

polipoprotein B-100 (APOB)重组蛋白Apo B-100 (Apo B-48)蛋白
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  • ¥1344 - 10872
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  • CSB-EP001918HUb0
  • 2025年07月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human Apolipoprotein B-100 (APOB), partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥10872.0
    规格:100μg产品价格:¥2514.0
    规格:20μg产品价格:¥1344.0

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    基因名:

    APOB

    Uniprot No.:

    P04114

    别名:

    Apo B-100 (Apo B-48)

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Partial

    来源:

    E.coli

    分子量:

    14.7 kDa

    表达区域:

    28-127aa

    氨基酸序列:

    EEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMS

    蛋白标签:

    N-terminal 10xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    In Stock Protein

    Biological_Activity:

    /

    Research Areas:

    Epigenetics and Nuclear Signaling

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    "Molecular cloning of human LDL apolipoprotein B cDNA. Evidence for more than one gene per haploid genome." Shoulders C.C., Myant N.B., Sidoli A., Rodriguez J.C., Cortese C., Baralle F.E., Cortese R. Atherosclerosis 58:277-289(1985)

    Function:

    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

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    图标文献和实验
    该产品被引用文献
    "Molecular cloning of human LDL apolipoprotein B cDNA. Evidence for more than one gene per haploid genome." Shoulders C.C., Myant N.B., Sidoli A., Rodriguez J.C., Cortese C., Baralle F.E., Cortese R. Atherosclerosis 58:277-289(1985)
    相关实验
    • 载脂蛋白(apolipoprotein B)

      人的载脂蛋白有两种表达形式: apoB-48和apoB-100。该基因的序列在肝、肠组织细胞中是相同的,但产物不同。在肝细胞中,产物为4563个氨基酸残基的肽:apoB-100; 但在肠细胞中,产物为只含2152个氨基酸残基的apoB-48, 缺失了apoB-100的N端同LDL受体结合的结构域。原因是在mRNA水平上将2153位的谷氨酰胺的密码子CAA中的C编辑成为U,形成一个终止密码子�UAA,结果使蛋白质的合成提前终止,合成一个新的相对分子质量约为250kDa的蛋白

    • Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B

      subfractions. In isolated, perfused rat livers inhibition of hepatic lipase with antibodies or removal of HL by heparin perfusion reduces the uptake of chylomicron remnants (13 ). Finally, recent studies with cultured hepatoma cells over-expressing human HL

    • 免疫透射比浊法

      of apolipoprotein AI and B,Clin Chem,1991,37:1676 10.陶义训主编.免疫学和免疫学检验,北京:人民卫生出版社,1989 11.刘秉文.载脂蛋白AⅠ及B测定标准化问题第一次调查报告.生命的化学,1991,11:41 12.钱士匀,周新.光谱技术.见:王霞文主编.临床生化检验技术.南京:南京大学出版,1995,1~15 13.贺小玲等.双试剂双波长检测载脂蛋白AⅠ,B-100、CⅡ的方法学探讨,中国实验临床免疫学杂志,1997 14.Edward SG

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    APOB/APOB蛋白Recombinant Human Apolipoprotein B-100 (APOB)重组蛋白Apo B-100 (Apo B-48)蛋白
    ¥1344 - 10872