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TNNC2/TNNC2蛋白Recombinant Human

Troponin C, skeletal muscle (TNNC2)重组蛋白TNNC2; Troponin C; skeletal muscle蛋白
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  • ¥1344 - 10872
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  • CSB-EP024010HU
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human Troponin C, skeletal muscle (TNNC2)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥10872.0
    规格:100μg产品价格:¥2514.0
    规格:20μg产品价格:¥1344.0

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    基因名:

    TNNC2

    Uniprot No.:

    P02585

    别名:

    TNNC2; Troponin C; skeletal muscle

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Full Length

    来源:

    E.coli

    分子量:

    45 kDa

    表达区域:

    1-160aa

    氨基酸序列:

    TDQQAEARSYLSEEMIAEFKAAFDMFDADGGGDISVKELGTVMRMLGQTPTKEELDAIIEEVDEDGSGTIDFEEFLVMMVRQMKEDAKGKSEEELAECFRIFDRNADGYIDPEELAEIFRASGEHVTDEEIESLMKDGDKNNDGRIDFDEFLKMMEGVQ

    蛋白标签:

    N-terminal GST-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    13-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    Developed Protein

    Biological_Activity:

    /

    Research Areas:

    Signal Transduction

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    "Differential expression of slow and fast skeletal muscle troponin C. Slow skeletal muscle troponin C is expressed in human fibroblasts." Gahlmann R., Wade R., Gunning R., Kedes L. J. Mol. Biol. 201:379-391(1988)

    Function:

    Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components

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    图标文献和实验
    该产品被引用文献
    "Differential expression of slow and fast skeletal muscle troponin C. Slow skeletal muscle troponin C is expressed in human fibroblasts." Gahlmann R., Wade R., Gunning R., Kedes L. J. Mol. Biol. 201:379-391(1988)
    相关实验
    • troponin蛋白纯化 Protein purification: troponins

      regulatory mechanism for muscle contration.       Material Source: skeletal muscle ether powder Equipment: • centrifuge • Cibaron Blue-sephacryl column (5 x 50 cm) • DEAE-sephadex A-50 column (2.5 x 25 cm) • CM-sephadex C-50-120 column

    • Determination of Muscle Fiber Type in Rodents

      . Type IIx myosin heavy chain transcripts are expressed in type IIb fibers of human skeletal muscle. Am. J. Physiol. 267:C1723‐C1728.    Smith, B. 1964. The Localization

    • 蛋白复性(二)-基因重组蛋白质的体外复性

      X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    TNNC2/TNNC2蛋白Recombinant Human Troponin C, skeletal muscle (TNNC2)重组蛋白TNNC2; Troponin C; skeletal muscle蛋白
    ¥1344 - 10872