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- 英文名:
Fluorescent Dye 700-I Succinimidyl Ester
- 库存:
Fluorescent Dye 700-I Succinimidyl Ester
- 供应商:
艾美捷
- CAS号:
Fluorescent Dye 700-I Succinimidyl Ester
- 规格:
1mg
产品名称:http://www.amyjet.com/news/abnova-updates.shtml-Fluorescent Dye 700-I Succinimidyl Ester
产品货号:U0256-E
产品规格:1mg
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保存建议:http://www.amyjet.com/news/abnova-updates.shtml-Fluorescent Dye 700-I Succinimidyl Ester待更新
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点击:http://www.amyjet.com/news/abnova-updates.shtml-Fluorescent Dye 700-I Succinimidyl Ester
关键词:生化试剂,荧光染料700-I琥珀酰亚胺酯,待更新
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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 6.While vortexing,add 166 µl (8.31 mg)CFSE stock solution to 1 ml of buffer 2. 7.Mix dye solution immediately with actin solution,by gentle pipeting or stirring.Incubate at room temperature for 1 hr. 8.Centrifuge in a 50Ti rotor at 40,000 rpm,4℃ for 1 hr
Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
.Take a 10-20μl aliquot and dilute with 400μl buffer.Read OD at 556 nm and calculate dye concentration based on the molar extinction coefficient of 50,000.D/P should be around 0.75 per tubulin monomer.
abnormalities related to pathological states, or for studying the effects of extrinsic factors. In the present chapter, we describe the use of the intracellular fluorescent dye carboxyfluorescein succinimidyl ester (CFSE) to monitor cell
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