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- 详细信息
- 技术资料
- 英文名：
Tide Quencher™ 5.1WS succinimidyl ester [TQ5.1WS SE]
- 库存：
50
- 供应商：
广州市左克生物科技发展有限公司
- 规格：
1mg
| Ex (nm) | - | Em (nm) | - |
| 分子量 | N/A | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称：荧光淬灭剂Tide Quencher™ 5.1WS succinimidyl ester [TQ5.1WS SE]
储存条件：保存在冰箱-15℃干燥
保质期：12个月
产品物理化学光谱特性
外观：固体
分子量：1137.43
溶剂：DMSO
Ex(nm): N/A
Em(nm): N/A
产品介绍
Tide Quencher™ 5.1WS (TQ5.1WS) 是一种非荧光分子，旨在有效猝灭常见 NIR 荧光团的荧光，例如 Cy5、Cy5.5、Alexa Fluor® 647、Alexa Fluor® 647、iFluor® 647 和 iFluor® 680。它是TQ5和QSY 21和BHQ3的改进版本。 TQ5.1WS 被设计为具有 (a) 的优质淬火剂。更强的吸收，和（b）淬火效率更高。 Tide Quencher™ 5.1WS 琥珀酰亚胺酯主要用于氨基修饰寡核苷酸和肽的 N 末端或赖氨酸残基的后标记。它可用于聚合酶链式反应 (PCR)、实时 PCR 和 DNA 测序等技术。在这些应用中，荧光信号用于监测特定 DNA 序列的扩增或检测。 TQ5.1WS 会淬灭荧光信号，直到发生特定事件（例如 DNA 链分离或引物延伸），从而导致可检测和定量的荧光增加。基于荧光共振能量转移 (FRET) 的检测广泛用于检测和测量样品中特定分子的存在。它们涉及使用荧光分子（荧光团）和猝灭剂分子，例如 TQ5.1WS。荧光团在被特定波长的光激发时发射光，而猝灭剂分子吸收这种发射的光，有效地“猝灭”荧光信号。
溶解方案(溶剂以说明书为准)
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 87.917 µL | 439.587 µL | 879.175 µL | 4.396 mL | 8.792 mL |
| 5 mM | 17.583 µL | 87.917 µL | 175.835 µL | 879.175 µL | 1.758 mL |
| 10 mM | 8.792 µL | 43.959 µL | 87.917 µL | 439.587 µL | 879.175 µL |
参考文献
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Journal: FEMS microbiology letters (2023)
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Authors: Boukhatem, Hadjer and Durel, Beatrice and Raimbault, Manon and Laurent, Audrey and Olivier, Nicolas
Journal: Biomedical optics express (2023): 550-558
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
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