Fluorescent Dye 488-I Maleimid

Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities

to the amber stop codon TAG. Proteins were purified with high yield and subsequently labeled with the hydroxylamine derivative of Alexa Fluor� 488 functioning as a fluorescent donor dye. The proteins were then labeled via maleimide coupling chemistry

Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells

  Reagents Cells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as control. Hoechst 33342 stock solution (1mg/ml) (see recipe) 12 X 75 mm culture tubes Vortex

Fluorochrome Absorption and Emission

which can be conveniently tuned to emit light in the 550-650 nm range. (In a dye laser, the lasing medium is a solution of fluorescent dye excited by a pump laser, usually an ion laser.) Texas Red ™ , available conjugated to avidin, can be excited by an argon-krypton mixed