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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Tubulin beta chain (TUBB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Tubulin beta chain (TUBB)品名简称:
Recombinant Human TUBB protein货号:
CSB-EP025318HU规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
TUBBUniprot No.:
P07437别名:
Tubulin beta-5 chain种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
50.6 kDa表达区域:
1-444aa氨基酸序列:
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
Signal Transduction请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验实验助攻 | WB 实验 (Western Blot) 杂带太多?原因就这几个!
产品推荐 Beta Actin 抗体 (HRP)(YA4634) www.medchemexpress.cn/antibody/beta-actin-antibody-hrp-conjugated.html 常用的内参抗体之一,可检测 Beta Actin 总蛋白的内源水平。 alpha Tubulin 抗体 (YA625) www.medchemexpress.cn/antibody/alpha-tubulin-rabbit-mab.html 可检测 alpha-tubulin 总蛋白的内源水平
【原创】转载Western Blot 内参选择攻略(美季生物技术有限公司)http://www.majorbio.cn/NewsInfo.asp?id=289
Strip缓冲液洗掉膜上的抗体,重新进行内参蛋白的抗体温育、显色检测。第三种,当目的蛋白的分子量大小与选用的内参蛋白分子量大小相差比较明显情况下,可以在转膜后预染,根据蛋白质Marker的大小将膜剪为大分子量和小分子量两部分,使内参蛋白与目的蛋白分开,然后两块膜分别与内参蛋白抗体以及目的蛋白抗体进行温育,二抗温育以及显色。 常用的蛋白质内参有GAPDH(glyceraldehyde-3-phosphate dehydrogenase)和细胞骨架蛋白beta-actin或beta-tubulin
体系,显色体系等。beta Actin、beta Tubulin、GAPDH、Lamin B1、PCNA 等已作为成熟的内参运用到试验中,下面就大致介绍下如何选择内参:样本来源● 如果是哺乳动物的组织或细胞,主要以 beta Actin、beta Tubulin、GAPDH、Lamin B1、PCNA 为代表;● 如果是植物样本,主要以 actin、Rubisco 为代表。目的蛋白定位● 全细胞蛋白和细胞质:beta Actin、beta Tubulin、GAPDH 等;● 细胞核蛋白: PCNA
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