Recombinant Human TUBB4/Tubuli

Recombinant Human TUBB4/Tubulin beta-4 chain  (CG33)

 

产品说明(Description)

Recombinant Human Tubulin Beta-4A Chain is produced by our E.coli expression system and the target gene encoding Met1-Ala444 is expressed with a 6His tag at the N-terminus. 

Accession #: P04350

Known as: Tubulin Beta-4A Chain; Tubulin 5 Beta; Tubulin Beta-4 Chain; TUBB4A; TUBB4; TUBB5

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM PB，150mM NaCl,pH7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Tubulin Beta-4A Chain (TUBB4A) is a cytoplasmic peptide containing 444 amino acids. TUBB4A is a member of the Tubulin family. Tubulin is the major constituent of microtubules. Tubulin is a dimer composed of one alpha and one beta tubulin molecule; there are many forms of beta tubulins, Beta II and Beta IV Tubulin are ubiquitously expressed. Beta-III Tubulin, also known as Tubulin Beta-4, is regarded as a neuron-specific marker. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY