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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human N-myc proto-oncogene protein (MYCN)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥12060.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2820.0 |
| 规格: | 20μg | 产品价格: | ¥1500.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
MYCNUniprot No.:
P04198别名:
Class E basic helix-loop-helix protein 37 ;bHLHe37种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
Yeast分子量:
51.6 kDa表达区域:
1-464aa氨基酸序列:
MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
May function as a transcription factor.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
CancerReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
Nucleotide sequence of the human N-myc gene.Stanton L.W., Schwab M., Bishop J.M.Proc. Natl. Acad. Sci. U.S.A. 83:1772-1776(1986)Function:
Positively regulates the transcription of MYCNOS in neuroblastoma cells.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验肿瘤发生新机制!Nature 子刊发文:lncRNA 通过结合核糖体相关蛋白参与神经母细胞瘤的发生机制
癌症是儿童疾病死亡的最常见原因,其中神经母细胞瘤约占儿童癌症相关死亡的 15%。约 25% 的人类神经母细胞瘤组织中存在 MYCN 癌基因扩增和相应的 N-Myc 癌蛋白的过表达,且与患者预后不良相关。研究发现,Myc 癌蛋白可以通过调节基因转录来促进细胞增殖和肿瘤发生。长期以来,人们对 LncRNA 的研究尚浅,认为其不具有任何生物学功能,只是作为转录过程的副产物而存在。随着测序技术的发展和普及,LncRNA 被作为新的研究热点。最新的研究表明,LncRNA 可以通过调节染色质结构、基因
【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
,Bohm G, lilie H, et al. Folding proteins. In:Creighton T E ed. Protein Function: A Practical Approach, 2nd.New York: IRL,1997,57-99 [7]CowleyDT, MackinRB. Expression, purification and charaterization of recombinant human proinsulin. FEBS Lett,1997
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