Lamc1|Lamc1蛋白|Lamc1小鼠蛋白|Lamc1

Protein Therapeutics: Mouse, Humanized, and Human Antibodies

Antibodies play a vital role in immune defense against pathogens. They are globular glycoproteins produced by plasma cells in response to the antigenic stimulation of B-lymphocytes. Their circulation in blood and lymph contributes to the humoral

【原创】Protein A Sepharose 之进化篇。。。

洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下

Exploration of the Visual System: Part 1: Dissection of the Mouse Eye for RNA, Protein, and Histological Analyses

Alternate Protocol 1: RNA Preparation from Pure RPE Cells Basic Protocol 4: Protein Preparation from Eye Tissues Basic Protocol 5: Fixation, Inclusion, and Cryosection of the Mouse Eye